Interaction of proteins with spherical polyelectrolyte brushes

By (author) Katja Henzler

Image coming soon

Publisher: Logos Verlag Berlin

Subjects: Earth & Life Sciences 

Language: English

Paperback (Published)

(June 2011)

ISBN: 9783832528669

5.71 x 8.27 inches

Price: $66.00

Out of stock

Add to cart -
back order

Spherical polyelectrolyte brushes are a novel class of carrier particles for the immobilization of proteins. These particles consist of a polystyrene core onto which long chains of polyelectrolytes are grafted. A high uptake of proteins can be achieved if the ionic strength is low, while both compounds carry an overall negative charge. No adsorption takes place at higher ionic strength. This thesis provides a deeper insight in the adsorption process of proteins onto polyelectrolyte brushes. Here, the kinetics of the adsorption process is investigated by the combination of time resolved small angle X-ray scattering and rapid stopped flow mixing. The adsorption kinetics was described experimentally and theoretically. Furthermore, the thermodynamics of the adsorption process was investigated by isothermal titration calorimetry. The combination of experimental finding and theoretical considerations identify the counterion release force as the main driving force for the adsorption. Counterions from the brush layer and the protein surface are released at low ionic strength and the free energy of the system will be decreased. Furthermore, the behavior of the proteins in the adsorbed state was investigated by the combination of several analytical techniques. These provide an in-depth understanding of the kinetic activity, the protein structure and the location of the adsorbed protein molecules in the polyelectrolyte layer.

  • By (author) Katja Henzler

Similar Books

Book cover: Ecological Conversion and the Murray River Ecological Conversion and the Murray River

Towards a Changed Relation with a Great River System

By (author) Emmanuel D. Bautista

Book cover: Global Legal Pluralism and Rights of Nature Global Legal Pluralism and Rights of Nature

Volume 151

Edited by Daniel Bonilla Maldonado Edited by Ralf Michaels

Book cover: Gravity Gravity

Life's Ups and Downs

By (author) David Howe

Book cover: Naming Pathology from Antiquity to the Early Modern Period Naming Pathology from Antiquity to the Early Modern Period

Volume 20

edited by Alessandra Foscati and Michele Goyens

Book cover: Tabule Resolute Tabule Resolute

The Central European Type of Alfonsine Tables

Volume 4

By (author) Petr Hadrava By (author) Alena Hadravová

Book cover: Zoo Quest for a Dragon Zoo Quest for a Dragon

By (author) David Attenborough

Book cover: Zoo Quest to Guyana Zoo Quest to Guyana

By (author) David Attenborough

Book cover: Jan Schröder: Recht als Wissenschaft Jan Schröder: Recht als Wissenschaft

Ein Kommentar

Edited by Marietta Auer Edited by Ralf Seinecke Edited by Stefan Vogenauer

Book cover: „Our house is on fire“ „Our house is on fire“

Theater / Drama / Theatertext und Klimakrise

Volume 5

Volume editor Andreas Englhart Volume editor Artur Pelka Volume editor Hannah von Sass

Book cover: A Mosaic of Recollections A Mosaic of Recollections

Memoirs of an Archaeologist

By (author) David S. Neal

Book cover: Architecture Is Construction Architecture Is Construction

Global Award for Sustainable Architecture

By (author) Marie-Hélène Contal By (author) Jana Revedin By (author) Marta Maccaglia

Book cover: Disaster Resilience and Normalization Disaster Resilience and Normalization

Facing Facts

By (author) Naomi Zack

Book cover: Gardens, Flowers, and Fruit Gardens, Flowers, and Fruit

Proceedings of the Oxford Symposium on Food and Cookery 2024

edited by Mark McWilliams

Book cover: Giovanni Pontano, Commentationes in centum sententiis Ptolemaei Giovanni Pontano, Commentationes in centum sententiis Ptolemaei

A Critical Edition

Volume 5

Edited by Michele Rinaldi

Book cover: History of the Physiologus in Early Medieval England History of the Physiologus in Early Medieval England

Volume 5

By (author) Mercedes Salvador-Bello

Book cover: Mastia Tarseion Revisited Mastia Tarseion Revisited

The Geographical Limits of Polybius Second Romano-Carthaginian Treaty

by Luis Silva Reneses

Book cover: Materiality of Air Materiality of Air

Edited by Tatiana Konrad

Book cover: Medical Theory and Practice in Early Modern Italy Medical Theory and Practice in Early Modern Italy

Volume 9

Edited by Sandra Cavallo Edited by John Henderson

Book cover: Nicolaus Copernicus in the Culture of Memory Nicolaus Copernicus in the Culture of Memory

Sedimentation of Knowledge

Volume 8

edited by Adam F. Kola and Wojciech Piasek

Book cover: Orthodox Christianity and the Study of Nature Orthodox Christianity and the Study of Nature

Histories of Interaction

Volume 4

Edited by Kostas Tampakis Edited by Ronald L. Numbers

Book cover: Science and Diplomacy Science and Diplomacy

The Max Planck Society in International Politics (1945-2000)

by Carola Sachse

Book cover: Science and Sensibility Science and Sensibility

From the Heavens Above to the Earth Below

by David Howe

Book cover: The Archaeology of Irrigation Technology and Water Management in the Islamic World The Archaeology of Irrigation Technology and Water Management in the Islamic World

Proceedings of the Bahrain Conference, January 2024

Edited by Timothy Insoll Edited by Rachel Maclean Edited by Salman Almahari

Book cover: The People of Early Winchester The People of Early Winchester

Volume 9.1

Edited by Caroline M. Stuckert

Book cover: Waterscapes Archaeology Waterscapes Archaeology

Multi-Scalar Human-Environment Interactions in Coastal Lagoons

Edited by Alexandra Bivolaru Edited by Daniela Cottica Edited by Christophe Morhange

Book cover: La fabuleuse histoire du monde vegetal La fabuleuse histoire du monde vegetal

by Alain Bonjean

Book cover: Betschart, Berichtende Formen Betschart, Berichtende Formen

Wie Bildungswelten erzeugt werden

by Benjamin Betschart

Book cover: Conrad Gessner, Fossilienbuch Conrad Gessner, Fossilienbuch

Ubersetzt und herausgegeben von Petra Schierl

by Conrad Gessner and edited by Petra Schierl and contributions by Urs B. Leu, Walter…

Book cover: Das Klima im Lichte der Geistes- und Sozialwissenschaften Das Klima im Lichte der Geistes- und Sozialwissenschaften

edited by Alexis Metzger, translated by Karin Becker

Book cover: Leben am Fluss Leben am Fluss

Der Alpenrhein von Reichenau bis Buchs 1790-2020

by Luca Locher

Book cover: Nouvelles normes, surtout pas de panique! / Neue Normen, nur keine Panik! Nouvelles normes, surtout pas de panique! / Neue Normen, nur keine Panik!

Reguler les pollutions en Suisse a l'age industriel / Umweltverschmutzung und Regulierung in der industrialisierten Schweiz

edited by Nicolas Chachereau, Alexandre Elsig, Sabine Pitteloud and Tiphaine Robert

Book cover: Rohstoffmacht Russland Rohstoffmacht Russland

Eine globale Energiegeschichte

by Jeronim Perovic

Book cover: Soft Seats and Blisters Soft Seats and Blisters

Zur Geschichte von Parkways, Panoramastrassen, Trails und Wanderwegen in der Hochmoderne

Volume 30

by Dirk Thomaschke

Book cover: Universitare Munzsammlungen im deutschsprachigen Raum Universitare Munzsammlungen im deutschsprachigen Raum

Geschichte, Gegenwart und Zukunft

edited by Martin Mulsow, Johannes Wienand and Katharina Martin

Book cover: A History of the Congress of Roman Frontier Studies 1949-2024 A History of the Congress of Roman Frontier Studies 1949-2024

By (author) David J. Breeze By (author) Tatiana Ivleva By (author) Rebecca H. Jones By…

Book cover: A Map of the Body, a Map of the Mind: Visualising Geographical Knowledge in the Roman World A Map of the Body, a Map of the Mind: Visualising Geographical Knowledge in the Roman World

By (author) Iain Ferris

Book cover: A View from the Herd A View from the Herd

Cattle, Sheep, Goats, and Pigs in Pharaonic Egypt: A Primer for Egyptologists and Archaeologists

Volume 5

By (author) Richard W. Redding

Book cover: Adrift at Sea Adrift at Sea

By (author) Melissa Publishing House Edited by George Papatheodorou

Book cover: An Irish Civil War Dugout: Tormore Cave, County Sligo An Irish Civil War Dugout: Tormore Cave, County Sligo

Archaeology, History, Memory

By (author) Marion Dowd By (author) Robert Mulraney By (author) James Bonsall

Book cover: Archaeology and Geology of Ancient Egyptian Stones Archaeology and Geology of Ancient Egyptian Stones

By (author) James A. Harrell

Book cover: Communicating about Climate Change Communicating about Climate Change

Volume 39

Edited by K. Davidse Edited by A.-M. Simon-Vandenbergen

Book cover: Development of a System for Fast Identification and Characterization of Biological Cells Development of a System for Fast Identification and Characterization of Biological Cells

Volume 11

By (author) Viviane Silva Teixeira