Optimization in HPLC

This handy guide provides detailed practical information for HPLC practitioners of all skill levels, enabling them to optimize the performace of their HPLC/UHPLC system for a wide range of analytical tasks.

PART 1: OPTIMIZATION STRATEGIES FOR DIFFERENT MODES AND USES OF HPLC
Working under regulatory constraints
Reverse phase gradient methods
2-Dimensional chromatography
HILIC
LC-MS
Biomolecules
Supercritical fluid chromatography
Chiral substances
Column selection for small molecules
Optimization based on analyte structure
 
PART 2: COMPUTER-AIDED OPTIMIZATION
ChromSword
DryLab
Fusion QbD
Quality by Design approaches to determine the robust range
 
PART 3: CURRENT CHALLENGES FOR HPLC USERS IN INDUSTRY
Big Chemistry
Big Analytical Service Providers
Small Analytical Service Providers
 
PART 4: CURRENT CHALLENGES FOR HPLC EQUIPMENT SUPPLIERS
Thermo Fisher Scientific
Agilent
Shimadzu
Waters
Knauer

• Edited by Stavros Kromidas